Quantitative Proteomics: of Chemically Excited Cultured Neurons - Ry Tweedie-cullen - Bøger - VDM Verlag Dr. Müller - 9783639262216 - 8. juni 2010
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Quantitative Proteomics: of Chemically Excited Cultured Neurons

Ry Tweedie-cullen

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Quantitative Proteomics: of Chemically Excited Cultured Neurons

Mammalian cells secrete a large number of proteins into the extracellular space, the composition and levels of which change as result of environmental factors. However, the identity of most of these secreted proteins has yet to be determined. Recent advances in proteomic technology now allow the isolation and identification of these secreted proteins. Available evidence suggests that under excitotoxic stress, neurons secrete signals that can alter the function of neurons with which they are connected via synapses or to which they are proximate. In this study we used primary neuronal cell cultures derived from the adult rat brain, and investigated the effect of chemical excitation on secreted proteins. The conditioned medium of these cultured cells was analysed using 2D-PAGE combined with MALDI-TOF mass spectrometry.

Medie Bøger     Paperback Bog   (Bog med blødt omslag og limet ryg)
Udgivet 8. juni 2010
ISBN13 9783639262216
Forlag VDM Verlag Dr. Müller
Antal sider 124
Mål 225 × 7 × 150 mm   ·   190 g
Sprog Engelsk