Fortæl dine venner om denne vare:
In Vitro Regeneration of Niger (Guizotia Abyssinica Cass.): Using Cotyledon and Hypocotyl Explants
Tesfaye Disasa Bitema
Bestilles fra fjernlager
In Vitro Regeneration of Niger (Guizotia Abyssinica Cass.): Using Cotyledon and Hypocotyl Explants
Tesfaye Disasa Bitema
Callus induction and shoot formation from hypocotyls and cotyledons of Guizotia abyssinica Cass. has been achieved. Six to eight-day-old hypocotyl segments and cotyledons were cultured on MS medium containing different concentrations of NAA, IAA and BAP. Among the various concentrations tested, 0.5 mg/l NAA in combination with 1 mg/l BAP was found to be the best for maximum callus induction of hypocotyl explants. Furthermore, 2 mg/l IAA in combination with 1 mg/l BAP was the best for callus induction of cotyledonary explants. Highest percentage of shoot formation (60%) was obtained when cotyledons were cultured on medium supplemented with 3.0 mg/l IAA in combination with 1.0 mg/ l BAP. Maximum number of shoots per explant (20.3) was obtained from medium containing 0.1 mg/l NAA in combination with 1 mg/l BAP. The types of explant, growth regulator combinations and genotypes were showed significant effect on shoot regeneration. The elongated shoots were successfully rooted on media supplemented with IBA at a concentration of 0.5 mg/l. The shoots were established in soil where 65% of them survived. Morphologically aberrant plants were not observed.
Medie | Bøger Paperback Bog (Bog med blødt omslag og limet ryg) |
Udgivet | 27. juni 2012 |
ISBN13 | 9783659168673 |
Forlag | LAP LAMBERT Academic Publishing |
Antal sider | 72 |
Mål | 150 × 4 × 226 mm · 117 g |
Sprog | Engelsk |
Se alt med Tesfaye Disasa Bitema ( f.eks. Paperback Bog )