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Enhancement of Xylitol Production in Candida Tropicalis: Enhanced Xylitol Production in Candida Tropicalis by Engineering Glycerol Metabolic Pathway & Pentose Phosphate Pathway
Irshad Ahmad
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Enhancement of Xylitol Production in Candida Tropicalis: Enhanced Xylitol Production in Candida Tropicalis by Engineering Glycerol Metabolic Pathway & Pentose Phosphate Pathway
Irshad Ahmad
In this study, gk gene was disrupted from xylitol dehydrogenase gene (XYL2) knockout C. tropicalis strain BSXDH-3. The resulting strain was incapable to grow on glycerol. The cells growth on glycerol was resumed by co-expression of gcy1, 2 and 3 genes from Scheffersomyces stipitis under the control of C. tropicalis glyceraldehydes-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed ?IA-3?, than in BSXDH-3. The pentose phosphate pathway (PPP) is a major basis of NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)-disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes, which respectively encode glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "PP", than in BSXDH-3. This is the first report of increased metabolic flux toward glycerol metabolic and PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production.
Medie | Bøger Paperback Bog (Bog med blødt omslag og limet ryg) |
Udgivet | 3. januar 2012 |
ISBN13 | 9783847335016 |
Forlag | LAP LAMBERT Academic Publishing |
Antal sider | 108 |
Mål | 150 × 7 × 226 mm · 167 g |
Sprog | Engelsk |